20% blasts in bone marrow required for diagnosis (WHO).
The following clonal cytogenetic abnormalities considered to have AML regardless of the blast percentage:
FAB classification of AML:
Type |
Name |
Associated Chromosomal Abnormality |
Associated Immunophenotype on Flow Cytometry |
M0 |
Acute undifferentiated leukemia |
|
CD13, CD33, CD34, CD65, HLA-DR, CD117 |
M1 |
Myeloblastic leukemia. No maturation Morphology exactly similar to M2 |
CD13, CD33, CD34, CD65, HLA-DR, CD117 |
|
M2 |
Myeloblastic leukemia with differentiation |
t(6;9)(p23;q34) |
CD33, CD34, CD65, HLA-DR |
M3 |
Acute Promyelocytic Leukemia (APL). Cytoplasm contains multiple rods. |
CD15, CD33, CD65 |
|
M3V |
Microgranular varient of APL. |
t(15;17)(q22:q21) |
|
M4 |
Both myelocytic and monocytic differentiation present in varying proportions |
MLL (11q23) t(6;9)(p23;q34) |
CD11b, CD13, CD14, CD15, CD33, CD65, CD36, CD4, HLA-DR |
M4EOS |
|
|
|
M5 |
Acute Monoblastic Leukemia |
MLL (11q23) |
CD11b, CD13, CD15, CD33, CD65, CD36, CD4, HLA-DR |
M6 |
Acute Erythroleukemia |
|
CD33, CD65, Glycophorin A |
M7 |
Acute Megakaryocytic Leukemia |
CD33, CD41, CD61, CD42, CD36, CD2 |
Link:
Classification of Pediatric Myeloid
Malignancies at the National Cancer Institute
